脑缺血再灌注后大脑皮质环氧化酶-2的表达及川芎嗪的干预作用

Expression of cyclooxygenase 2 in the cerebral cortex and protective effect of tetramethylpyrazine after cerebral ischemic-reperfusion

目的:探讨环氧化酶-2(COX-2)在脑缺血再灌注损伤后的表达及其作用,为治疗缺血性脑病提供实验依据。方法:以线栓法制作大鼠大脑中动脉阻塞的局灶性脑缺血再灌注模型,采用免疫组织化学和神经行为相结合的方法,观测缺血再灌注侧大脑皮质内COX-2表达和神经功能的变化。结果:脑缺血再灌注组COX-2免疫阳性神经元在再灌注6h后较假手术组明显增多,并随着再灌注时间的延长逐渐增加,再灌注24h后达高峰;与模型组比较,川芎嗪治疗组COX-2的表达明显减少。结论:脑缺血再灌注后COX-2的表达较正常明显增多,是导致脑缺血再灌注损伤的因素之一,川芎嗪对大鼠脑缺血再灌注损伤有保护作用。

Objective： To investigate the role and expression of cyclooxygenase 2 （COX-2） after cerebral ischemic-reperfusion injury and provide basis of treatment. Methods： The focal cerebral ischemic-reperfusion model was established with thread embolisb of the middle cerebral artery. Immunohistochemistry and neurological evaluation was performed to examine the expression of COX-2 in temporal-parietal cortex and neurological function disorders. Results： COX-2 immunoreative neurons were increased significantly from 6 h after reperfusion. With the increase of I/R time, the expression of COX-2 increased, and reached its peak at I2h/R24h; Compared with model group, in tetramethylpyrazine treatment group, the expression of COX-2 were markedly downregulated. Conclusion： The expression of COX2 increased in cerebral isehemic-reperfusion group, indicating COX-2 may play an important role in the mechanisms of cerebral ischemic-reperfusion injury; TMP may have a neuroprotective effect by inhibiting the expression of COX-2.