糖尿病大鼠视网膜N-甲基-D-天门冬氨酸受体表达的变化

Changes of N-methyl-D-aspartate receptor expression in diabetic rat retina

目的:观察糖尿病不同时期视网膜N-甲基-D-天门冬氨酸(NMDA)受体各亚基的表达。方法:采用雄性SD大鼠腹腔内注射链脲佐菌素建立糖尿病大鼠模型,分别于4、8、12周灌注,取眼球、冷冻切片;免疫组织化学、原位杂交方法检测NMDA受体NR1、NR2A、NR2B亚基表达的变化;用RS IMAGE软件进行图像分析。结果:NR1、NR2A、NR2B基因及其蛋白表达在糖尿病第4周时开始升高,至12周升高具有统计学意义,特别是NR1的变化更为显著。结论:NMDA受体各亚基的高表达参与了糖尿病视网膜病变。

Objective： The purpose of this study is to observe the changes of expressions of N-methyl-D-aspartate receptor （NMDAR） subunits NR1, NR2A and NR2B gene in diabetic rat retina. Methods： Diabetes mellitus （DM） was induced by intraperitoneal injection of streptozotocin in Sprague-Dawley rats. After 4, 8, and 16 weeks, the eyeballs were removed for examination. Expression of NR1, NR2A, NR2B in rat retina was examined by in situ hybridization and immunohistochemis- tory. The staining results were analyzed by RS IMAGE software. Results： In DM group, the expression of NR1, NR2A, NR2B in rat retina was significantly increased in comparison with that in the control group. Conclusion： The upper expression of NR1, NR2A, NR2B in streptozotocin-induced diabetic rat retina is one of the reasons of diabetic retinopathy.