阳虚体质者内分泌及免疫功能变化

Changes of endocrine and immune function in subjects of yang deficiency constitution

目的:观察阳虚体质者内分泌功能和环核苷酸系统及免疫功能的变化,探索阳虚质特征、成因与生理生化指标的关联性。方法:在临床流行病学调查的基础上,按阳虚质和平和质的诊断标准筛选阳虚质60例和平和质50例,清晨8:00～9:00空腹抽取静脉血8 ml,3000r/min离心5min分离血清,—70℃冰箱中保存,采用酶联免疫吸附检测(enzyme-linked immunosorbent assay,ELISA)法测定阳虚质及平和质血清皮质酮、皮质醇、促肾上腺皮质激素(adrenocorticotrophic hormone,ACTH)、环腺苷酸(cyclic adenosine monophosphate,cAMP)、环鸟苷酸(cyclic guanosine monophosphate,cGMP)、游离三碘甲状腺原氨酸(free triiodothyronine,FT_3)、游离甲状腺素(free thyroxine,FT_4)、促甲状腺素(thyrotropic-stimulating hormone,TSH)、白细胞介素1β(interleukin-1β,IL-1β)和白细胞介素2(interleukin-2,IL-2)水平,计算cAMP/cGMP比值,并对阳虚质与平和质上述指标之间的差异进行分析。结果:与平和质比较,阳虚质血清皮质酮、cAMP/cGMP比值、IL-1β和TSH含量较高,而血清皮质醇、ACTH、cGMP及FT_4含量则较低。结论:阳虚质与下丘脑-垂体-肾上腺轴、下丘脑-垂体-甲状腺轴功能减退,及环核苷酸系统和免疫功能紊乱具有一定的关联性。

Objective： To investigate the changes of endocrine, cyclic nucleotide and immune systems in subjects of yang deficiency constitution, and to explore the relationship among characteristics and causes of yang deficiency constitution, the physiological and biochemical parameters. Methods： Based on the diagnostic criteria for the clinical epidemiological investigation, sixty subjects of yang deficiency constitution and fifty of normal constitution were selected. Eight milliliters venous blood were taken from overnight fasted subjects at 8：00-9：00. The sera were obtained by centrifugatiorl of the blood at the speed of 3 000 r/min for 5 minutes, and they were stored at --70 ℃ until use. The serum levels of corticosterone, cortisol, adrenocorticotrophic hormone （ACTH）, cyclic adenosine monophosphate （cAMP）, cyclic guanosine monophosphate （cGMP）, free triiodothyronine （ FT3 ）, free thyroxine （ FT4 ）, thyrotropicstimulating hormone （TSH）, interleukin-1β and interleukin-2 were measured by enzyme-linked immunosorbent assay; the cAMP/cGMP ratio was also computed; and the differences of the above indexes were compared between the two types of subjects. Results： The serum levels of corticosterone, interleukin-1β, TSH and cAMP/cGMP ratio of yang deficiency constitution significantly increased as compared with those of normal constitution, and the serum levels of cortisol, ACTH, cGMP and FT4 of yang deficiency constitution significantly decreased in comparison with those of normal constitution. Conclusion： Subjects of yang deficiency constitution may be not only related to hypothalamic-pituitary-adrenal axis and hypothalamic-pituitary-thyroid axis, but also related to the functional disorders of cyclic nucleotide and immune systems.