CO_2超临界流体萃取前胡药材中白花前胡甲素

Extraction of praeruptorin A from Radix Peucedani by supercritical fluid extaction-CO_2

目的:采用CO_2超临界流体萃取法提取前胡药材中的白花前胡甲素。方法:采用正交实验设计法优选前胡中白花前胡甲素的最佳萃取条件,并与传统提取方法作比较。结果:以白花前胡甲素的提取量为指标,最佳萃取条件为萃取温度60℃、萃取压力20 MPa、萃取时间3h。加入夹带剂乙醇后白花前胡甲素提取量增加,且超临界流体萃取法明显高于水煎煮和加热回流法。结论:CO_2超临界流体萃取法提取前胡药材中白花前胡甲素的方法可行、可靠。

Objective： To extract praeruptorin A from Radix Peucedani by supercritical fluid extraction （SFE）-CO2. Methods： After preliminary experiment, three main factors were acquired that could influence the result of SFE-CO2, including the time, pressure and temperature of the extraction. The optimal extraction process was carried out on orthogonal design, and SFE-CO2 was compared with the traditional methods. Results： In the extraction of the praeruptorin A, the best extraction conditions were 60 ℃, 20 MPa, and duration for three hours. As cosolvent alcohol was added, the amount of extraction of the praeruptorin A increased, and the amount of SFE-CO2 extraction was higher than those of decoction and heating reflux. Conclusion： The SFE-CO2 in extracting praeruptorin A from Radix Peucedani is feasible and reliable.