IL-1β在缺血预处理对大鼠肾脏冷缺血再灌注损伤中第二窗保护作用的实验研究

Role of IL-1β in the second window of protection of ischemic preconditioning against renal cold ischemia/reperfusion injury in rats

目的:探讨IL-1β在缺血预处理对大鼠肾脏冷缺血再灌注损伤模型中第二窗保护的作用。方法:36只SD大鼠制备成肾脏冷缺血再灌注损伤模型,随机分为假手术组(A组)、冷缺血再灌注组(B组)、缺血预处理组(C组)。免疫组化观察IL-1β表达,测定肾功能变化,TUNEL法检测肾组织细胞凋亡情况。结果:B组、C组血浆尿素氮、肌酐测定值均>A组(P<0.01),而C组<B组(P<0.01);B组、C组IL-1β表达及原位细胞凋亡指数均>A组(P<0.01),而C组<B组(P<0.01);肾组织损伤的病理分级显著减轻(P<0.01)。结论:缺血预处理对肾脏冷缺血再灌注损伤第二窗具有保护作用,其机制可能与IL-1β表达减少有关。

Objective To investigate the role of IL-1β in the second window of protection of renal ischemic preconditioning against cold ischemia/reperfusion injury in rats. Methods Thirty-six SD rats with cold ischemia/reperfusion injury were randomly divided into： the sham operated group （group A）, cold ischemia/reperfusion group（group B）, and ischemic and reperfusion group （group C）. The expression of IL-1β was observed with immunohistochemistry. The level of renal function, cells apoptosis and histologic examination were all detected. Results Blood urea nitrogen and creatinine levels in group B and C were significantly higher than those in group C（P〈0.01）, and lower in group C than those in group B（P〈0.01）. IL-1β and TUNEL positive cells all increased in group B and C as compared with those in group A（P〈0.01）. Group C showed a less pathological change and a minor renal impairment（P〈0.01）. Conclusion Ischemic preconditioning has a protective effect on the second window of protection of renal cold ischemia/reperfusion injury through decreasing IL-1β expression.