肝癌组织中β-葡萄糖醛酸酶mRNA与临床病理因素的关系

Relationship between the expression of β-glucuronidase mRNA in hepatocellular carcinoma tissue and the clinicopathologic factors

目的探讨肝癌组织中B．葡萄糖醛酸酶（β-glucuronidase，β-G）mRNA的表达与临床病理因素的关系。方法采用RT-PCR法检测25例肝癌组织及10例正常肝组织中β-GmRNA的表达，并分析肝癌组织中β-GmRNA与临床病理因素的关系。结果患者的年龄、肿瘤大小、AFP水平不影响肝癌组织中β-G mRNA的表达。β-G mRNA的表达在有门静脉癌栓以及淋巴结转移者中明显高于无门静脉癌栓以及淋巴结转移者（t=7．857，9．341，P〈0．05）。结论β-G mRNA的表达与肝癌患者有无门静脉癌栓及淋巴结转移等临床病理因素密切相关，β-G可能在肝癌细胞的侵袭和转移过程中起一定的作用。

Objective To investigate the relationship between the expression of β-glucuronidase mRNA in hepatocellular carcinoma （HCC） tissue and the clinicopathologic factors. Methods The expressions of β-glucu-ronidase mRNA in 25 samples of HCC tissue and 10 samples of normal hepatic tissue were detected by RT-PCR, and the relationship between β-glucuronidase mRNA in HCC tissue and clinieopathologic factors was analyzed. Results The expression of β-glucuronidase mRNA in HCC tissue was not influenced by the age of patient, tumor size and alpba-fetoprotein level. The expression of β-glucuronidase mRNA in patients with portal vein tumor thrombus or lymph node metastasis was significantly higher than those without （t=7.857, 9. 341, P〈0.05 ）. Conclusions The expression of β-glucuronidase mRNA is closely related to the existence of portal vein tumor thrombus and lymph node metastasis. β-glucuronidase may play a role in the invasion and metastasis of HCC.