脉冲磁场对马尾松愈伤组织生长和生理生化特性的影响

Effect of Pulsed Magnetic Field on the Growth,Physiological and Biochemical Characteristics of Pinus massoniana Callus

以马尾松愈伤组织为试验材料,通过不同脉冲前沿宽度和处理时间的磁场处理,观察了马尾松愈伤组织在磁场处理后的生长变化,探讨了磁场处理对马尾松愈伤组织外部形态、可溶性蛋白和可溶性糖含量、保护酶活性和细胞膜透性的影响。结果表明:(1)脉冲磁场处理后,马尾松愈伤组织的鲜质量生长与对照相比有明显增加,其趋势为先缓慢上升,再急速下降;(2)脉冲磁场处理后,可溶性蛋白的含量、可溶性糖的含量与对照相比都有增加的趋势,当脉冲前沿宽度为5 ms、处理时间为8 h时,马尾松愈伤组织可溶性蛋白和可溶性糖的含量增加到峰值;(3)脉冲磁场处理后,与对照相比,由POD、SOD、CAT构成的保护酶系统的活性随脉冲前沿宽度、处理时间的增加而增强,在脉冲前沿宽度为5 ms、处理时间为8 h时,三种酶活性达到一定的峰值后又出现不同程度的下降;(4)在一定的脉冲前沿宽度和处理时间内,马尾松愈伤组织细胞膜仍可保持较强的稳定性,马尾松愈伤组织细胞对脉冲磁场具有一定的抗性和耐性。

The present research used Pinus massoniana callus as the experiment material. P. massoniana callus were treated with different pulse rise time and treating time, then the growth condition of P. massoniana callus was observed, the exterior shape, the content of soluble protein and soluble sugar and the activity of protective enzymes of P. massoniana callus were also been discussed. The experiment results were as follows ： （ 1 ） After treated with pulsed magnetic field, the fresh weight of P. massoniana callus increased obviously compared with the control group. The trend of its development was increasing slowly then decreasing sharply. （2） The treatment of pulsed magnetic field on the P. massoniana callus could increase the contents of soluble protein and soluble sugar. And those parameters increased to the highest level at the pulse rise time of 5 ms and treating time of 8 h. （3） After treated with pulsed magnetic field, the activity of protective enzyme system comprised of peroxidase （POD）, superoxide dismutase（ SOD）, catalase（CAT） increased with the improvement of pulse rise time and treating time. The activities of POD, SOD, CAT increased to the highest level at the pulse rise time of 5 ms and treating time of 8 h and then decreased with different degree. （4） At a certain pulse rise time and treating time, the cell membrane of P. massoniana callus could still maintain a relatively strong stability, indicating that ceils of P. massoniana callus had a certain resistance and endurance against pulsed magnetic field.