SALL4及BMI-1在急性白血病中的表达

Expression of SALL4 and BMI-1 mRNA in Acute Leukemia

本研究旨在探讨SALL4及BMI-1基因在急性白血病患者中的表达及其临床意义。运用逆转录-聚合酶链反应(RT-PCR)半定量检测62例急性白血病患者和10例对照者(非恶性血液病患者)骨髓细胞中SALL4及BMI-1的mRNA的表达水平。结果表明:①SALL4mRNA在除M3及T-ALL外的急性白血病中均高表达,在髓系白血病中表达显著高于B淋巴细胞白血病(p=0.022,<0.05);在对照者中9例不表达,1例低表达;②SALL4mRNA在初治AL中表达较对照组明显增高,完全缓解组的表达明显下降,与初治组有显著差异(p<0.05);复发组的表达也明显增高,且略高于初治组患者(p=0.414);③BMI-1除在M3型中也高表达外与SALL4的表达规律一致,两者表达呈显著正相关(r=0.684,p<0.01)。结论:SALL4及BMI-1在急性白血病中异常高表达,影响白血病的发生和发展,它可作为急性白血病的发病、复发及预后判断的可能指标。

This study was aimed to investigate the expression of SALL4 and BMI-1 mRNA in acute leukemia （AL） and its clinical significance, mRNA expression levels of SALL4 and BMI-1 in 62 AL patients and 10 controls were measured by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）. The results showed that （1） the expression of SALL4 mRNA were up-regulated in AL （ except M3 and T-ALL）, and the expression of of SALL4 mRNA in AML was significantly higher than that in B-ALL （p 〈 0.05 ） ; expression was negative in 9 out of 10 controls, and expression level of SALL4 mRNA in one case was low; （2） the expression of SALL4 in de novo AL was higher than that in controls, which significantly decreased at complete remission（CR）. The difference between CR group and de novo group was statistically significant （p 〈 0.05 ） . In relapsed patients, the expression of SALL4 increased, slightly higher than that in de novo AL group（p 〉0.05） ; （3） the expression pattern of BMI-1 was the same to that of SALL4 except the up-regulation in M3 , and the expression of BMI-1 was positively correlated with that of SALL4 in acute leukemia（ r=0. 684, p 〈0.01 ）. It is concluded that SALL4 and BMI-1 expressions are up-regulated significantly in acute leukemia, which may contribute to the development of leukemia, thus become an important index for evaluation of development, relapse and prognosis in acute leukemia.