免疫表型结合间期原位杂交法检测多发性骨髓瘤细胞遗传学异常

Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for the Investigation of Cytogenetic Aberrations of Multiple Myeloma

本研究旨在建立骨髓涂片荧光免疫表型结合间期原位杂交技术(FICTION),为检测多发性骨髓瘤(MM)患者的分子细胞遗传学提供新方法。以骨髓涂片为载体,通过标记FITC的抗CD138抗体筛选浆细胞(PC),比较筛选的PC比例与形态学检测的PC比例之间的差异。同时采用8号染色体着丝粒探针行间期荧光原位杂交技术,检测PC的8号染色体异常情况。结果表明:骨髓涂片上荧光抗体筛选的PC比例与骨髓形态学检测的PC比例相比无统计学差异(p>0.05),9例患者中4例(44%)有8号染色体异常,其中8号染色体单体(-8)3例(33%),8号染色体三倍体(+8)1例(11%)。结论:骨髓涂片FICTION技术具有简便、特异、准确的特点,可用于MM分子遗传学异常的研究。

This study was aimed to establish the technique of fluorescence immunophenotyping and interphase cytogenetics as a tool for the investigation of neoplasms （FICTION） used to smear of bone marrow, so as to develop a new technique for detection of the molecular cytogenetic abnormalities in multiple myeloma （MM）. By using the bone marrow smear as the cartier and the anti-CD138 antibody linked with FITC, direct fluorescence staining was applied to mark plasma cells （PCs） and differences were compared in the proportion of both PCs marked by fluorescence staining and PCs detected in morphology. At the same time, the chromosome 8 centromere probe was used in interphase fluorescence in situ hybridization （I-FISH） for detection of the chromosome 8 abnormalities in PCs marked by fluorescence staining. The results showed that there was no significant difference between the proportions of both PCs marked by fluorescence staining and PCs detected in morphology on smear （p 〉 0.05 ）. 4 out of 9 patients （44 % ） had the chromosome 8 abnormalities, including 3 cases with - 8 （33%） and one case with ＋ 8 （ 11% ）. It is concluded that the FICTION technique on the basis of bone marrow smear is characterized by convenience, specificity and accuracy. Therefore, it can be used for molecular cytogenetic research in MM.