外周血血细胞膜表面组织因子在脑梗塞患者的表达及活性的研究

Expression and Activity of Membrane Surface Tissue Factor in Peripheral Blood Cells of Patients with Cerebral Infarction

为了探讨外周血中单核细胞和血小板膜表面组织因子(TF)在脑梗塞患者的表达、活性变化及其临床意义,采用流式细胞术(FCM)检测25例脑梗塞患者外周血单核细胞及血小板膜表面TF的阳性表达率,采用发色底物法检测外周血单核细胞及血小板膜表面TF的活性,并与24例正常对照者进行比较。结果表明:脑梗塞患者外周血中单核细胞和血小板膜表面TF表达率明显高于正常对照(p<0.01),单核细胞和血小板膜表面TF活性亦明显高于正常对照(p<0.01)。结论:TF是凝血途径的启动因子,脑梗塞患者血细胞膜表面TF的表达及活性增强,提示血细胞来源的TF可能参与脑梗塞疾病中病理血栓的形成。

This study was aimed to investigate the expression and activity of membrane surface tissue factor （TF） of monocytes and platelets in peripheral blood cells from patients with cerebral infarction and their clinical significance. The TF expressions in monocytes and platelets from 25 patients with cerebral infarction were detected by flow cytometry, the TF activity was detected by chromogenic reaction method, and compared with 24 normal people used as control. The results showed that the TF expressions of monocytes and platelets in peripheral blood cells from patients with cerebral infarction were significantly higher than that in normal controls（p 〈 0.01 ）, and TF activity was also higher in patients than that in controls（p 〈 0.01 ）. In conclusion, the expression and activity of membrane surface in patients with cerebral infarction were enhanced, the hematocyte-derived tissue factor as a trigger in coagulation pathway is involved in pathological thrombosis in patients with cerebral infarction.