单侧迷路切除术后大鼠前庭神经核γ-氨基丁酸A受体β1亚型表达

Expression change of GABAA receptor subunits of the vestibular nuclear complex following unilateral labyrinthectomy in rat

目的观察单侧迷路切除术后大鼠前庭神经核复合体内γ-氨基丁酸(gamma-aminobutyric acid,GABA)A受体β1亚型的表达变化。方法成年雄性Wistar大鼠48只,分为实验组(36只)和对照组(12只),前者破坏单侧迷路,对照组手术方式相同但保持迷路完好。单侧迷路切除术后,分别用免疫组织化学法和免疫印迹法按照上述分组方式,检测不同存活时间(术后1、3、7天)组动物前庭神经复合体内GABAA受体β1亚型的表达变化。结果术后1、3、7夭组大鼠左右两侧比较及与对照组比较差异均无显著性意义。结论在前庭代偿的早期,前庭神经复合体内GABAA受体β1亚型表达无变化。

OBJECTIVE To investigate whether vestibular compensation following unilateral vestibular labyrinthectomy （UL） is associated with changes in the expression of gamma-aminobutyric acid （GABA） A receptor subunits βl of vestibular nuclear complex （VNC） in rat. METHODS Western blot and immunohistochemistry were used to quantify the expression of GABA A receptor subunits /γl in the VNC at ld, 3d and 7d following unilateral vestibular labyrinthectomy and control group in rat. RESULTS There were no significant differences in the protein expression of the/β1 subunit within the ipsilateral and contralateral VNC at any time after unilateral vestibular labyrinthectomy compared to that in control group. CONCLUSION The results suggest that expression of GABAA receptor β1 subunits in the VNC be not involved in the early stage of the vestibular compensation process.