白细胞介素-4对巨噬细胞缺氧诱导促有丝分裂因子分泌的影响

Effect of IL-4 on the expression of hypoxia-induced mitogenic factor of macrophages

目的:探讨白细胞介素-4(IL-4)对巨噬细胞缺氧诱导促有丝分裂因子(HIMF)分泌及HIMF对血管平滑肌细胞增殖的影响。方法:用不同剂量的重组IL-4刺激培养的巨噬细胞,以逆转录聚合酶链反应(RT-PCR)检测巨噬细胞HIMF mRNA表达,荧光免疫组化激光共聚焦显微镜检测巨噬细胞HIMF蛋白表达;用终浓度分别为3×10-6mmol/L,9×10-6mmol/L,2.7×10-5mmol/L的重组HIMF刺激培养的平滑肌细胞,以3H-胸腺嘧啶核苷(3H-TdR)掺入法测定HIMF对平滑肌细胞增殖的影响。结果:Th2型细胞因子IL-4刺激巨噬细胞,HIMF mRNA及其蛋白明显表达(P<0.01);浓度为3×10-6mmol/L,9×10-6mmol/L,2.7×10-5mmol/L的重组HIMF明显促进平滑肌细胞增殖(与对照组比较P<0.01),且随着HIMF剂量的增加,促增殖作用增强。结论:Th2型细胞因子IL-4能刺激巨噬细胞表达HIMF mRNA及其蛋白,HIMF能促进体外培养的血管平滑肌细胞增殖。

Objective： To explore the effect of IL-4 on the expression of hypoxia-induced mitogenic factor （HIMF） of macrophages and HIMF on the proliferation of vascular smooth muscle cell （VSMC）. Methods： Cultured macrophages collected from mouse peritonaeum were stimulated by different dose of recombinant IL- 4, the expression of HIMFmRNA and protein were detected by RT-PCR and laser confocal microscopy. The cultural VSMCs were stimulated with HIMF at different final concentrations of 3×10^-4mmol/L,9×10^-4mmol/L and 2.7×10^-5 mmol/L respectively. Then the effect of HIMF on the proliferation of VSMCs was detected by 3H--TdR. Results： When IL-4 stimulated macrophages, HIMFmRNA and protein expressed significantly （P〈0.01） ; HIMF promoted the proliferation of smooth muscle cell in a dose-dependent manner （HIMF groups compared with cantrol groups, P〈0.01）.Conclusion.. IL-4 significantly induced expression of HIMFmRNA and protein in macrophages; HIMF promoted the proliferation of smooth muscle cells.