PEG-PLGA纳米粒子介导反基因寡核苷酸体外抗前列腺癌作用研究

The effects of anti-gene oligonucleotides loaded PEG-PLGA nanoparticles on prostate cancer in vitro

目的验证包载反雄激素受体基因三螺旋形成寡核苷酸(TFO)的聚乙二醇-聚乳酸聚乙醇酸共聚物(PEG-PLGA)纳米粒子(TFO-NPs)对前列腺癌细胞的生长抑制作用。方法采用改良自乳化溶剂挥发法制备TFO-NPs并进行体外物化表征。将TFO-NPs、脂质体包载的TFO(TFO-Lip)和裸TFO分别转染体外培养的LNCaP细胞,倒置荧光显微镜观察转染效率,MTT法检测细胞增殖活性,RT-PCR和Westernblot方法检测AR基因表达。结果TFO-NPs平均粒径128nm,载药量为1.02%,包封率为72.28%,在体外具有缓释作用。LNCaP细胞对TFO-NPs和TFO-Lip的摄取率显著高于裸TFO。TFO-NPs和TFO-Lip对LNCaP细胞的抑制率分别为(54.5%±6.2%)和(50.6%±6.1%)显著高于裸TFO,(7.8%±0.8%)(均P<0.05)。TFO-NPs和TFO-Lip处理组LNCaP细胞的雄激素受体表达水平显著低于裸TFO处理组。TFO-NPs和TFO-Lip处理组的转染效率、细胞抑制率和雄激素受体表达水平差异均无显著性。结论成功制备了TFO-NPs,为反基因治疗前列腺癌的研究提供了有效基因载体。

Objective To evaluate the inhibitory effects of anti-androgen receptor gene triplexforming oligonucleotides （TFO） loaded nanoparticles （TFO-NPs） on prostate cancer cells in vitro. Methods Poly （ethylene glycol）-poly （lactide-co-glycolide） （PEG-PLGA） was used to prepare TFO-NPs by modified spontaneous emulsion solvent diffusion method and the morphology, encapsulation rate, drug loading efficiency and drug released characteristics of TFO-NPs were identified. Then TFO-NPs, TFO loaded liposome （TFO-Lip） and naked TFO were transfected into the cultured LNCaP cells. The transfection efficiency was observed by an inverted florescent microscope. The cellular proliferation was detected by MTT assay. The expression of androgen receptor gene was examined by RT-PCR. and Western blot assay, Results The TFO-NPs were spherical, uniform, with mean diameter of 128nm. The drug loading efficiency and the encapsulation rate of TFO-NPs were 1.02% and 72.28% respectively. The release test in vitro showed significant sustained release. The cellular uptake rate of either TFO-NPs or TFO-Lip was significantly higher than that of naked TFO. The inhibitory rate of either TFO-NPs （54.5%±6.2%） or TFO-Lip （50.6%±6.1%） for cellular proliferation was also significantly higher than that of naked TFO （7.8%±0.8%） （all P〈0.01）. The androgen receptor expression levels of LNCaP cells induced by either TFO-NPs or TFO- Lip were markedly lower than those induced by naked TFO. There was no significant difference in the transfection efficiency, the inhibitory rate and the androgen receptor expression levels between TFO-NPs treatment group and TFO-Lip treatment group. Conclusions The TFO-NPs are prepared successfully, and may be an effective gene carrier for further study of anti-gene therapy on prostate cancer.