HPLC测定妇炎平胶囊中苦参碱的含量

HPLC Determination of Matrine in Fuyanping Capsules

目的建立妇炎平胶囊中苦参碱的含量测定方法。方法采用高效液相色谱法,色谱柱为Kromasil C18(250mm×4.6mm,5μm);磷酸盐缓冲液(pH6.8)-乙腈-甲醇(66∶17∶17)为流动相;检测波长为220nm;流速1.0ml/min。结果在0.400～2.000μg范围内呈良好的线性关系(r=0.9997),平均回收率为99.6%,RSD为0.42%。结论本法快速简便,结果准确可靠。

Aim Todevelop a method for the determination of matrine in Fuyanping Capsules. Methods HPLC was used. The column was： KromasilCls （250mm × 4.6mm,5um） ; the mobile phase was： phsphate buffer（ pH 6.8）- acetonitrile-methanol（66： 17：17 ） ;the flow rate was： 1.0ml/min and the wavelength was： 220nm. Results the linear range was0. 400 -2. 000ug（ r = 0. 999 7 ） ;the mean recovery rate was 99.6% and RSD was 0.42%. Conclusion The method is rapid, simple and accurate.