高效液相色谱法测定人血中氢溴酸右美沙芬浓度

Content determination of dextromethorphan hydrobromide in human plasma by HPLC

目的研究氢溴酸右美沙芬在健康志愿者体内的血药浓度。方法采用高效液相色谱法测定8例健康受试者口服右美沙芬糖浆和片剂后不同时间血浆中的美沙芬浓度。结果右美沙芬在5--500 ng/ml浓度范围内呈良好线性关系（r=0.9965）。结论本法精密,准确,可用于氢溴酸右美沙芬的药动学研究。

Objective To study the concentration of dextromethorphan hydrobromide in human plasma. Methods An oral does of tablets and syrup were given to eight healthy volunteers in a randomized crossover design,respectively, the drug concentrations were determined by the HPLC. Results The dextromethorphan hydrobromide was a good linear,within a concentration range of 5 -- 500 ng/ml, r = 0. 9965. Conclusion The method is accurate and precise, it is usable for the study of clinical dextromethorphan hydrobromide pharmacokinetics.