TNF-α、IL-6、IL-8与溃疡性结肠炎严重程度相关研究

The severity correlation of TNF-α,IL-6 and IL-8 levels in plasma in patients with ulcerative colitis and their clinical significances

目的观察血浆TNF-α、IL-6及IL-8与溃疡性结肠炎(UC)严重程度的相关性,探讨炎性细胞因子在UC发病中的作用。方法采用酶联免疫吸附试验(ELISA法)检测不同严重程度溃疡性结肠炎患者56例及正常对照组血清TNF-α、IL-6及IL-8的水平。分别比较活动期溃疡性结肠炎患者与缓解期、正常对照组的TNF-α、IL-6及IL-8的水平的差异;比较缓解期患者与正常对照组的差异;比较重度溃疡性结肠炎患者与轻、中度患者血清TNF-α、IL-6及IL-8的水平的差异。结果活动期溃疡性结肠炎患者血清TNF-α、IL-6及IL-8的水平明显高于缓解期和正常对照组(P<0.05);缓解期患者血清TNF-α、IL-6及IL-8的水平与正常对照组相比差异无统计学意义(P>0.05);重度溃疡性结肠炎患者血清TNF-α、IL-6及IL-8的水平高于轻、中度组(P<0.05)。结论溃疡性结肠炎患者存在严重的细胞免疫功能紊乱;TNF-α、IL-6及IL-8在溃疡性结肠炎的发生和病情发展中起着重要作用;测定血清TNF-α、IL-6及IL-8的水平可反映溃疡性结肠炎的病情程度。

Objective To observe the severity correlation of TNF-α,IL-6 and IL-8 levels in plasma in patients with ulcerative colitis and investigate mechanism of ulcerative colitis. Methods TNF-α,IL-6 and IL- 8 levels in plasma were measured with ELISA methods in 56 patients with ulcerative colitis and 20 normal per- sons as a control group. The active and catabolic phases of ulcerative colitis were compared and the difference were compared among slight, moderate and sever groups. Results The level of serum TNF-α, IL-6 and IL-8 in active ulcerative colitis was much more higher than catabolic ulcerative colitis and contrast group （ P 〈 0.05 ） ; The level had no significant differences between catabolic ulcerative cohtis and contrast group（ P 〉 0.05 ）. The level in severe group was higher than that in moderate and slight group （ P 〈 0. 05 ）. Conclusions There is a sever disorders of cellular immunity in the patients with active ulcerative colitis. TNF-α, IL-6 and IL-8 play the very important roles in the genesis and development of ulcerative colitis. The measurement of TNF-α, IL-6 and IL-8 levels in plasma may contribute to predict the severity of ulcerative colitis.