阿仑磷酸钠和辛伐他汀对体外培养成骨细胞的影响

Effect of alendronate sodium and simvastatin on osteoblasts cultured in vitro

目的探讨辛伐他汀和阿仑磷酸钠对体外培养成骨细胞的影响。方法分离3周龄成骨细胞,体外培养时以辛伐他汀和阿仑磷酸钠刺激,观察成骨细胞碱性磷酸酶水平和成骨细胞增殖率。结果辛伐他汀、阿仑磷酸钠可以促进成骨细胞增殖,增加碱性磷酸酶的分泌,二者共同作用后作用更显著。结论辛伐他汀和阿仑磷酸钠能促进成骨细胞增殖和分泌碱性磷酸酶,可以联合作用促进骨形成。

Objective To study the effect of alendronate sodium and simvastatin on osteoblasts cultured in vitro. Methods Three weeks old SD rats osteoblasts were isolated, osteoblasts were stimulated with alendronate and simvastatin, proliferation ratio and alkaline phosphatase （ALP） activity were assessed. Results Alendronate sodium and simvastatin not only promoted proliferation of osteoblasts, but also stimulated ALP activity, combina- tion of both drugs had a significant effect. Conclusion Alendronate sodium and simvastatin can promote prolifera- tion and ALP activity of osteoblasts, which could be combinated to promote the form of bone.