螺旋藻混合营养培养基配方的优化研究

Optimization of mixotrophism culture medium of S.platensis

采用单因素试验和L9(34)正交试验研究螺旋藻混合营养培养过程中碳源NaHCO3、氮源NaNO3、磷源K2HPO4的最佳配比。结果表明:当葡萄糖浓度为3 g/L时,NaHCO3 8.4 g/L、NaNO32.0 g/L、K2HPO4 0.1 g/L即可满足螺旋藻碳、氮、磷营养的需求,干质量达到1.60 g/L。

From the experiments of one-factor and the L9（3^4） orthogonal experiment, we studied the optimal ration among NaHCO3, NaNO3 and K2HPO4, so it is NaHCO3 8.4 g/L, NaNO3 2.0 g/L, K2HPO4 0.1 g/L when the glucose concentration is 30 g/L, and the dry weight is up to 1.60 g/L The optimal ration can demand the carbon, nitrogen and phosphorus of spirulina