电针天枢不同配穴对溃疡性结肠炎大鼠结肠黏膜形态学的影响

Influence of 4 Tianshu Prescriptions on Morphology of Colonic Mucosa of Rats with Ulcerative Colitis

目的筛选治疗UC活动期较佳的俞穴处方,以探讨电针天枢不同配穴对溃疡性结肠炎(UC)结肠黏膜形态学的影响。方法将Wistar大鼠随机分为正常组、模型组、电针天枢组、电针天枢+中脘组、电针天枢+上巨虚组、电针天枢+中脘+上巨虚组。冰乙酸灌肠造模。观察各组大鼠的结肠黏膜损伤情况、组织学及超微结构变化。结果模型组黏膜损伤较重,各电针治疗组黏膜损伤较轻,与模型组相比均有不同程度的良性改变。从组织学评分来看,正常组和天枢+中脘+上巨虚组与模型组相比有非常显著差异(P<0.001);天枢组、天枢+中脘组、天枢+上巨虚组与模型组亦有显著差异(P<0.01或0.05)。结论电针天枢不同配穴对UC大鼠结肠黏膜有明显的保护作用,以天枢、中脘、上巨虚三穴配伍疗效最优。

Objective： To observe morphology of colonic mucosa with electro - acupuncturing（EA） 4 ＂ Tianshu＂ prescriptions on the UC rats by selecting a sati sfactory acupoint prescription for ulcerative colitis（UC） . Methods： 60 wistar rats were randomly divided into six group： normal control group, model control group, EA＂ Tianshu＂ group, EA ＂ Tianshu＂ and＂ Zhongwan＂ group, EA＂ Tianshu＂ and＂ Shangjuxu＂ group, EA＂ Tianshu＂, ＂Zhongwan＂ and ＂Shangjuxu＂ group. The model was induced by enema of acetic acid（AA）. After the treatment, the change of macrography, histology and ultrastructure of colonic mucosa was observed. Results： Compared with model control group, mucosa of all EA groups was significantly less injuried and more rehabilitated, especially of EA＂ Tianshu＂, ＂Zhongwan＂ and ＂Shangjuxu＂ group. Conclusion： EA Tianshu with different prescriptions has definitely protective effect on the UC rats＇ colonic mucosa and EA＂ Tianshu＂eooperated with＂ Zhongwan＂and＂ Shangjuxu＂ is the most effective one.