多频大幅脉冲传感系统监测3种食源性致病菌的生长趋势

A multi-frequency large amplitude pulse sensor system to monitor the growth of food-borne pathogens

【目的】探究智能电子舌伏安法结合特定的统计分析系统是否适用于示踪食品致病性细菌生长情况的快速监测。【方法】利用智能电子舌——多频大幅脉冲传感系统的伏安法,监测液体培养基中3种食源性致病菌的16个时段生长情况所致液体基质变化过程的综合信息;结合主成分分析法对获得的复杂综合信息数据进行统计学分析,按获得的主成分得分图分析检测样品。【结果】监测能力强的电极、频率段分别是:金黄色葡萄球菌的为钨电极的100 Hz、铂电极的1 Hz、银电极的10 Hz和钛电极的10 Hz频率段;大肠杆菌O157:H7的为金电极的100 Hz、铂电极的1 Hz、钛电极的1 Hz和钨电极的100 Hz频率段;枯草芽孢杆菌的为钯电极的1、10和100 Hz 3个频率段。【结论】本试验首次用多频大幅脉冲传感系统伏安法结合主成分分析法,能够有效的监测样品细菌的生长情况,有望成为一种具有多种优点的新型检测细菌生长情况的快速监测系统。

[Objective] We explored whether a smart electronic tongue as a rapid technique, combining with specifically statistics analyze system, could monitor the growth of the three food-borne pathogens. [Methods] The smart electronic tongue, multi-frequency large amplitude pulse was based on voltammetry .We used it to detect the comprehensive information about a sample and the, change process of the medium in 16 periods of time. We analyzed the complex data information in statistics with principal component analysis. According to principal component scores plot, we analyzed the sample. [Results] The growth of Staphylococcus aureus in liquid medium could be monitored by tungsten electrode in 100 Hz frequency segment of multi-frequency large amplitude pulse voltammetry, platinum electrode in 1 Hz, and both silver electrode and titanium electrode in 10 Hz. The growth of Escherichia coli 0157：H7 could be measured by gold electrode in 100 Hz frequency segment, while platinum electrode in 1Hz, titanium electrode in 1Hz and tungsten electrode in 100 Hz Then, palladium electrode was efficient to Bacillus subtilis in 1 Hz, 10 Hz, 100 Hz. [Conclusion] We used the multi-frequency large amplitude pulse sensor system combining with principal component analysis for the first time. It has a promising future as a modern rapid analytical technology for detecting the growth of microbe.