三磷酸腺苷结合转运蛋白G超家族成员2基因在食管癌中的表达及意义

Expression of ATP-binding Cassette Transporter G2 Gene in Esophageal Carcinoma and its Significance

目的探讨三磷酸腺苷结合转运蛋白G超家族成员2(ATP-binding cassette transporer G2,ABCG2)基因在食管癌组织中的表达及其意义。方法采用RT-PCR方法检测44例原发性食管癌患者的癌组织、癌旁组织(距离癌2cm)及食管切缘正常黏膜(距离癌5cm以上)ABCG2的mRNA表达。结果ABCG2在食管癌原发灶组织中有较高程度的表达,其表达量为0.77±0.11,而正常食管黏膜中的表达量为0.66±0.12;其在食管癌中的表达显著高于正常黏膜(P<0.01)。ABCG2在低分化鳞癌中的表达显著高于中-高分化鳞癌(P<0.05)。食管癌中ABCG2在年龄和性别间表达无显著性差异(P>0.05)。结论ABCG2在食管癌组织中高表达可能在某种程度上参与了食管癌原发性耐药及化疗中多药耐药的形成。其可作为一种新的引起多药耐药的分子,指导临床上食管癌的化疗。

Objective To explore the expression of ABCG2 in esophageal carcinoma and its significance. Methods The expressions of ABCG2 mRNA were detected by RT-PCR in 44 cases of esophageal carcinoma, paired adjacent mueosa to esophageal carcinoma and normal esophageal mucosa at surgical margin of esophageal carcinoma tissues. Results The expression of ABCG2 in esophageal carcinoma was higher significantly than that in normal esophageal mueosa （P 〈 0.01 ）. The expression of ABCG2 in esophageal carcinoma was 0.77±0.11 and 0.66±0.12 in ormal mucosa. The expression of ABCG2 in poorly differentiated cancer was higher significantly than in moderately and well differentiated cancer （P 〈 0.05 ）. The expression of ABCG2 in esophageal carcinoma was not related to age and gender （P 〉 0.05 ）. Conclusion The high expression of ABCG2 may participate, to some extent,in the formation of esophageal muhidrug resistmlce, so it can be regarded as a new factor of multidrug resistance to guide the esophageal chemotherapy in clinic.