肺癌患者血清TNF-β和外周血T淋巴细胞亚群变化的研究

Study of Changes of Serum TNF-β and Peripheral Blood T Lymphocyte Subset in Patients with Lung Cancer

目的探讨肺癌患者血清肿瘤坏死因子β(TNF-β)及外周血T淋巴细胞亚群的变化。方法应用ELISA法对肺癌患者血清TNF-β以及采用流式细胞仪(FCM)对肺癌患者外周血T淋巴细胞亚群进行检测。结果肺癌患者血清TNF-β水平均明显低于正常对照组;而作为TNF-β的产生细胞T淋巴细胞(CD3+CD19-),特别是活化性T淋巴细胞(CD8+CD28+)均明显低于正常对照组。结论肺癌患者外周血T淋巴细胞亚群及血清TNF-β的水平均下降,且两者变化呈正相关,提示其与肺癌患者机体的免疫功能下降有关。

Objective To study the changes of serum TNF-β and T-lymphocytes in peripheral blood of patients with lung cancer. Methods Changes of serum TNF-β were detected by using ELISA techniques and T-lymphocyte subset in peripheral blood were measured by the flow cytometer in lung cancer patients. Results The level of serum TNF-β in lung cancer patients was obviously lower than that in healthy subjects. T Lymphocytes （CD3＋CD19-）, especially activated T lympbocytes （CD8＋CD28＋） producing TNF-β were obviously lower than those in healthy subjects. Conclusion The levels of T-lymphocyte in peripheral blood and serum TNF-β are lower in lung cancer patients, and their changes have a positive correlation, suggesting that these changes may be related to the decrease of the immunological function in lung cancer patients.