摘要
To investigate the effect of photodynamic therapy （PDT） with hematoporphrin monomethyl ether （HMME） on bovine immunodeficiency virus （BIV） can provide the basis theory for photoinactivation of human immunodeficiency virus （HIV）. To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-（4,5）-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide （MTT） with power density of 5 and 25 mW/cm^2 and energy density from 0.6 to 3 J/cm^2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.
To investigate the effect of photodynamic therapy （PDT） with hematoporphrin monomethyl ether （HMME） on bovine immunodeficiency virus （BIV） can provide the basis theory for photoinactivation of human immunodeficiency virus （HIV）. To assess the protection of HMME-PDT on the cell line Cf2Th infected with BIVR29 by 3-（4,5）-dimethylthiahiazol-2-yl-3,5-di-phenytetrazolium bromide （MTT） with power density of 5 and 25 mW/cm^2 and energy density from 0.6 to 3 J/cm^2. To observe the inhibition of membrane fusion using a new reporter cell line BIVE by fluorescence microscope. HMME-PDT has significant protectant effects on Cf2Th-BIVR29 with both power densities, especially in the group of high power density. Fluorescent microscope shows that there is no significant difference between the group of PDT and control, which means PDT could not inhibit the BIV-mediated membrane fusion.
基金
supported by the National Natural Science Foundation of China(No.60678047)
the Science Foundation of Tianjin(No.05YFJZJC02300)
