摘要
目的构建重组胶原矿化骨／BMP2活性多肽新型仿生骨修复材料，并评价其异位成骨能力。方法将48只成年SD大鼠随机分为4组，在A、B、C组的大鼠背部肌肉内分别植入含3、2、1mg BMP2活性多肽的重组胶原矿化骨；在D组的大鼠背部肌肉内植入单纯重组胶原矿化骨，于4、8和12周时间点将大鼠处死，经CT三维重建、组织学观察及钙含量测定检测植入材料的成骨情况。结果A、B、C组，在各时间点的CT三维重建、组织学观察及钙含量检测结果均表明其异位成骨能力优于D组，差异有统计学意义（P〈0．01），其中A、B两组的异位成骨能力优于C组，差异有统计学意义（P〈0．01）。结论BMP2活性多肽可以显著增强重组胶原矿化骨的骨诱导活性，这种骨诱导性存在一定的剂量效应关系。
Objective To prepare a new biomimetic bone matrix material with BMP2-derived peptide combined with bone based mineralized recombinant collagen and investigate its eetopie osteogenetic capacity. Methods Forty-eight adult Sprague-Dawley rats were divided into four groups randomly. Muscle of the rats＇ back in group A, group B and group C was implanted with bone based mineralized recombinant collagen combined with 3,2,1 mg BMP2-derived peptide, separately. On the week 4,8 and 12 after implantation, the rats were killed and the samples were harvested. Their osteogenic capability was detected by three-dimensional reconstruction of computed tomography, histological observation and content determination of calcium. Results Results Results of three-dimensional reconstruction of computed tomography, histological observation and content determination of calcium indicated that the osteogenie capability of group A, group B and group C was superior to group D with the difference being statistically significant （ P 〈 0.01 ）. The osteogenie capability of group A and group B was superior to group C with the difference being statistically significant （P 〈 0.01 ）. Conclusion BMP2-derived peptide can increase the osteoinduction of bone based mineralized recombinant collagen and the osteoinduetion has dose-effect relation to some extent.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第12期1655-1656,共2页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30470483）
国家高科技研究发展计划“863”重大项目（2006AA02A124）
