人类脐带源间充质干细胞细胞因子分泌及造血支持作用的研究

Cytokine production and hematopoiesis-supportive function of human umbilical cord mesenchymal stem cells

目的研究脐带源间充质干细胞（UC—MSC）细胞因子分泌特征及其对脐血源CD34^＋细胞的造血支持作用，并与骨髓源间充质干细胞（BM—MSC）相对照。方法RT—PCR法检测UC—MSC和BM—MSC细胞因子分泌。将脐血CD34^＋细胞分别接种于UC—MSC或BM—MSC滋养层共培养5周，收集贴壁与非贴壁细胞接种于标准甲基纤维素培养基，计数克隆形成细胞（CFC）。结果UC—MSC表达SCF、LIF、M—CSF、Flt-3、IL-6、GM—CSF、G—CSF、SDF-1和VEGFmRNA，不表达IL-3mRNA。与BM—MSC相比，二者表达细胞因子谱相似，但BM—MSC不表达GM—CSF和G—CSFmRNA。共培养5周计数CFC显示，BFU—E、GFU—GM和CFU—GEMM在CD刍细胞／UC—MSC（75．5±27．9，100．0±25．1，9．5±7．3）与CD34^＋细胞／BM—MSC（70．5±28．8，108．3±9．5，8．5±5.1）共培养体系差异无统计学意义（P〉0．05）。结论UC—MSC与BM—MSC具有相似的造血支持功能和细胞因子分泌谱。

Objective To investigate the cytokine spectrum and hematopoiesis-supportive function of umbilical cord derived mesenchymal stem cells （UC-MSC）, and compare with those of normal adult bone marrow derived mesenchymal stem cells （BM-MSC）. Methods The mRNA of cytokine production of UCMSC and BM-MSC were determined by reverse transeriptase polymerase chain reaetion（RT-PCR）analysis. To evaluate hematopoiesis supporting activity, cord blood （CB） CD34^＋ cells were co-cultured with UC-MSC or BM- MSC. Colony-forming cells （CFC） were determined after 5 weeks of culture. Results RT-PCR assay showed that UC-MSC had a eytokine spectrum very similar to that of BM-MSC, including expression of the mRNA of stem cell factor, leukemia inhibitor factor, macrophage colony stimulating factor, Flt3-1igand, interleukin-6, vascular endothelial growth factor and stromal derived factor-1, but UC-MCS additionally expressed mRNA of granulocyte macrophage and granulocyte colony-stimulating factors. After co-culture with CD34^＋ cord blood cells for 5 weeks, no significant difference in CFC was observed between the CD34^＋ cells/UC-MSC and CD34^＋ cells/BM-MSC co-cultures （P〉0.05）. Conclusion The cytokine spectrum and hematopoiesis-supponive function of UC-MSC are similar with that of BM-MSC.