BMI-1基因在白血病患者中的表达及其意义

BMI-1 gene expression and its significance in leukemia

目的探讨BMI-1基因在白血病中的表达及意义。方法应用反转录-聚合酶链式反应（RT—PCR）技术，检测BMI—1mRNA在慢性粒细胞白血病（CML）细胞株（K562），慢性粒细胞白血病（CML）、慢性淋巴细胞自血病（CLL）、急性自血病（AL）患者和对照组的骨髓单个核细胞（BMMNC）中的表达及临床意义。结果BMI-1基因在对照组中无表达；K562细胞株中呈阳性表达；CML组中的阳性率为15.4％，其中CML慢性期（CML-CP）与CML急变期（CML—AP）组阳性率比较差异有统计学意义（P〈0．05）；CLL组中无表达；AL组中阳性率为47．1％。结论BMI-1基因在白血病中存在较高的表达，且与疾病的转归相关，可能为向血病的靶向治疗提供新的位点.

Objective To investigate the expression and its significance of BMI-1 gene in leukemia patients. Methods BMI-1 gene level was assessed by reverse transcription polymerase chain reaction （RT- PCR） in acute leukemia patients, chronic myeloid leukemia （CML） patients, chronic lymphoblastic leukemia （CLL） patients, and bone marrow mononuelear cells（BMMNC） as normal controls, as well as leukemia cell line K562. The expression of BMI-1 gene in leukemia and the relationship was explored. Results The expression of BMI-1 gene was positive in leukemia cell line K562,but negative in 10 controls as well as in CLL. The BMI-1 gene was expressed in 15.4 % of CML patients and 47.1% of AL patients. The positive expression of BMI-1 gene in CML-CR was significantly lower than that in CML-BP （P 〈0.05）. Conclusion BMI-1 gene was over expressed in leukemia patients, and may be related to curative effect of the disease. It may be used for leukemia treatment targeting the provision of new srtes.