急性白血病Survivin和B7—1基因的表达及其临床意义

Expression and clinical significance of survivin and B7-1 genes in acute leukemia

目的研究Survivin和B7—1基因在急性白血病（AL）患者中的表达及临床意义。方法应用RT—PCR检测64例AL患者Survivin和B7—1mRNA的表达水平，14名健康人为对照，K562、HL-60细胞株为阳性对照。结果Survivin的阳性表达率高于健康对照，且在初治AL中的表达水平较健康对照组明显升高，治疗缓解后Survivin表达下降，复发时表达水平升高；同时患者B7—1基因的表达率于对照组没有差异，但半定量后的表达水平明显较正常低，且初发和缓解患者表达量差异无统计学意义；在初治AL患者中Survivin阳性患者缓解率低于表达阴性的患者。结论Survivin和B7—1基因表达的紊乱可能在AL的发病中起着重要作用，并与AL复发和预后相关。

Objective To investigate the expression of survivin and B7-1 mRNA in acute leukemia and the prognostic significance in AL. Methods The expression of survivin and B7-1 mRNA was measured in 64 AL patients with semi-quantity reverse transcription（RT）-PCR. 14 adults were tested as normal controls （NC） and K562, NB4 cell lines were also tested as positive controls. Results The positive rates of survivin was higher than that of normal controls（NC）. The expression of survivin in de novo AL was higher than that in NC, while it decreased in complete remission （CR） patients. In relapsed patients the expression of survivin increased again.Meanwhile the expression of B7-1 has no significient change, but the expression was lower than that in NC. In AL patients, the CR rate of positive survivin cases was lower than that of negative survivin cases. Conclusion Abnormal expression of survivin and BT-1 genes was related to pathogenesis and progression of AL and it can serve as a marker of relapse and poor prognosis in AL.