大蒜油固体脂质纳米粒中二硫化物和三硫化物在大鼠体内的组织分布

Tissue distribution of solid lipid nanoparticles loaded garlic oil in rats

目的:考察大蒜油固体脂质纳米粒(GO-SLN)中二硫化物(DADS)和三硫化物(DATS)在大鼠体内的组织分布。方法:建立了测定大鼠体内大蒜油中二硫化物(DADS)和三硫化物(DATS)的气相-电子捕获法,色谱条件:恒温110℃,检测器300℃,汽化室180℃,载气N2(纯度>99.999%),流速1.0 mL.min-1,分流比1∶10;尾吹60 mL.min-1;进样量1μL。并测定大鼠颈静脉注射GO-SLN和GO注射液后组织中的药物浓度。结果:此色谱条件下各组织的标准曲线、精密度等实验结果表明,该方法适于分析大鼠体内大蒜油中DADS和DATS含量。与GO注射液相比,GO-SLN在大鼠体内的分布特性有不同程度的改变,GO-SLN在各组织中分布均相对较高。结论:SLN能一定程度上提高药物的被动靶向性并延长药物在各组织中的作用时间。

Objective： To investigate the tissue distribution of the diallyl disulfide （DADS） and diallyl trisulfide （DATS） in solid lipid nanoparticles loaded garlic oil （GO-SLN） in rats. Method： The gas chromatography-electron capture detection （GC-ECD） metho;t was established to determined the DADS and DATS simultaneously in the biological samples of rats after administration of 0. 5 mL garlic oil injection or GO-SLN （containing about 10 mg garlic oil） via jugular vein cannula. The conditions for gas chromatographic separation were as follows. The oven temperature was set at 110 ℃ and maintained for 15 min. Temperatures at the injection port and detector were 180 ℃ and 300 ℃, respectively. Ultra-pure nitrogen （purity 〉 99. 999%, Shenyang Kerui Special Gases Co. Ltd. , China） was used as a carrier gas and made-up gas at flow-rates of 1 mL ·min^-1 and 60 mL · min^-1, respectively. All injections were carried out in the split injection mode with a split ratio of 1： 10. Result： The GC-ECD method was fit for determing the concentration of DADS and DATS in garlic oil. The distribution character of GO-SLN in rats had changed to some extent and the concentration of GO- SLN in tissues was higher than that of GO-Injection. Conclusion： The SLN can elevate the passive targeting of drugs and lengthen their action time in tissues.