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实时荧光PCR技术定量检测转Bt基因水稻的研究 被引量:4

Detecting of Bt transgenic rice by real-time quantitative PCR technique
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摘要 以转Bt基因的"克螟稻"为研究材料,通过使用特异的引物和荧光标记探针,以已知转基因成份含量的水稻样品为模板建立标准曲线,对转基因水稻的NOS和Bt外源基因进行了荧光定量检测分析。初步建立了转基因水稻定量检测的技术方法。 A quantitative method was developed for detecting Bt transgenic rice by real-time PCR with the specific primer and probe to amplify the endogenous gene SPS and exogenous nos and bt gene. The standard curve of Ct vs the genetically modified organism (GMO) content based on reference materials was generated and a linear regression equation was obtained to quantify Bt transgenic rice.
出处 《生物学杂志》 CAS CSCD 2008年第6期63-65,71,共4页 Journal of Biology
基金 安徽省自然科学基金项目(050430301)
关键词 实时荧光PCR 转BT基因水稻 定量检测 real-time PCR Bt transgenic rice quantitative detection
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共引文献388

同被引文献47

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