摘要
研究了在15L发酵罐中添加氟化钠对林可霉素合成的影响。结果表明,96h向培养基中添加100mg/L氟化钠,发酵液中林可霉素的效价达5795u/ml,比对照提高12.6%。进一步研究发现,氟化钠可以降低糖酵解途径中丙酮酸激酶的酶活力,使更多碳源流向磷酸戊糖途径,可为林可霉素合成提供更多前体,从而提高林可霉素产量。
The biosynthesis of lincomycin by addition of sodium fluoride to medium in 15L fermentor was studied. The result showed that the production achieved to 5795u/ml, which was increased by 12.6% over the control group, when 100mg/L sodium fluoride was added into the medium at 96h. The mechanism of sodium fluoride was revealed in further study, that is the activity ofpyruvate kinase in EMP was decreased, so more glucose flowed into HMP, which provided more precursors so that more lincomycin was obtained.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2008年第12期895-897,共3页
Chinese Journal of Pharmaceuticals
关键词
林可霉素
糖酵解抑制剂
氟化钠
酶活力
lincomycin
inhibitor of glycolytic pathway
sodium fluoride
enzyme activity
