不同饲料对军曹鱼组织消化酶活力的影响

Effects of Different Diets on Digestive Enzymatic Activities in Several Tissues of Juvenile Cobia(Rachycentron canadum)

比较研究了投喂冰鲜鱼和人工配合饲料的军曹鱼胃、前肠以及肝脏消化酶(蛋白酶、胰蛋白酶、淀粉酶和脂肪酶)活力。试验结果表明,(1)蛋白酶活力:前肠与肝脏的蛋白酶比活力,投喂配合饲料组显著高于冰鲜鱼组(P<0.05),而胃中的蛋白酶比活力以冰鲜鱼组显著高于配合饲料组(P<0.05);(2)胰蛋白酶活力:胃中胰蛋白酶比活力,冰鲜鱼组显著高于配合饲料组(P<0.05),前肠与肝脏的胰蛋白酶比活力配合饲料显著高于冰鲜鱼组(P<0.05);(3)淀粉酶活力:胃中的淀粉酶比活力,冰鲜鱼组显著高于配合饲料组(P<0.05),前肠的淀粉酶比活力则是配合饲料组显著高于冰鲜鱼组(P<0.05),两种饲料对军曹鱼肝脏淀粉酶比活力影响不显著(P>0.05);(4)脂肪酶活力:胃及前肠的脂肪酶比活力两种饲料间差异不显著(P>0.05),但肝脏的脂肪酶比活力则以配合饲料组显著高于冰鲜鱼组(P<0.05)。

The objective of the study was conducted to determine the effects of two diets（ ice fresh trash fish and formulated feed）on the activity of digestive enzymes（ protease, trypsin, amylase and lipase）in stomach, proximal intestines and liver of juvenile eobia（ Rachycentron canadum）. The diets were fed to the juveniles with initial mean weight of（ 10.79 ±2.12） g for 14 days indoor flow-through and aerated aquaria at a rate of 20 individuals per tank with triplication. The results showed that the juveniles fed the ice fresh trash fish had significantly higher protease activity in the stomach than the juveniles fed the formulated feed（ P 〈 0.05 ）, but the case were contrary in the proximal intestines and liver（P 〈 0.05 ）. There was significantly higher activity of trypsin in the stomach of the animals fed the ice fresh trash fish than that in the stomach of the animals fed the formulated feed（P 〈0.05）, but the case were contrary in the proximal intestines and liver（ P 〈 0. 05 ）. The significantly higher activity of lipase was found in the liver of the fish fed the ice fresh trash fish than that in the fish fed the formulated feed（P 〈 0.05 ）, and there was no significant difference in lipase activity in the stomach and the intestines in the fish fed the two diets（ P 〉 0.05 ）. The juveniles fed the ice fresh trash fish had significantly higher amylase activity in the stomach than the juveniles fed the formulated feed（P 〈0.05）. For the same enzyme, the case in the intestines was opposite to that in the stomach, and there was no significant difference in lipase activity in the liver in the fish fed the two diets （P 〉 0. 05）. The changes in the four enzymatic activities between the two groups in the experiment suggested that the different diets had different effect on activities of the digestive enzymes, and the enzymes activities showed tissue specificity for cobia juveniles.