野西瓜总皂苷诱导人肝癌细胞HepG-2凋亡的初步研究

Preliminary study on apoptosis in HepG-2 of human hepatoma cell line induced by CSS

[目的]探讨野西瓜总皂苷(CSS)对人肝癌HepG-2细胞的杀伤作用和诱导凋亡的作用。[方法]四甲基偶氮唑蓝(MTT)法研究CSS对HepG-2细胞的杀伤作用;荧光显微镜观察细胞凋亡形态;碘化吡啶(PI)单染经流式细胞仪检测CSS对HepG-2细胞周期及凋亡的影响。[结果]CSS对HepG-2细胞的生长增殖具有抑制作用;经CSS作用后,HepG-2细胞在出现特征性凋亡形态特征,并出现了G1期阻滞,G2期比例下降,高剂量组出现了G2期消失的现象,50mg/L剂量作用48h,凋亡细胞比率高达66.652%。[结论]CSS对人肝癌HepG-2有明显的杀伤和诱导凋亡作用,并出现细胞周期的改变。

[Objective] To discuss the killing and apoptosis-inducing effect of total alkaloid in the CSS （Capparis spinosa L. saponin, CSS） on human heptocarcinoma cell Line HepG-2. [Methods] The killing effect of the CSS on cell Line HepG-2 was observed by MTT method. Morphological observation of the HepG-2 cells was completed by fluorescence microscope. This test was signed to observe the changes of the cell cycle of HepG-2 affected by the CSS with PI single-staining. [Results] CSS had inhibiting effect on the proliferation of HepG-2 and its IC50 value was 46.16 ug/mL. Characteristic apoptosis appeared after the HepG-2 cells was affected with CSS, The cell cycle changed obviously that the progresses of cells cycle of G1 period and G2 period in high dosage group have been blocked, and the cellular proportion in G2 period is decreased by the action of CSS for 24h. The apoptosis percentage is increased to 66.652% in the 50ug/mL dosage group. [Conclusion] The CSS has obvious killing and apoptosis-inducing effect on human heptoearcinoma cell Line HepG-2 and the cell cycle changes have been observed.