组织胺对肠黏膜屏障保护作用的研究

Research and Analysis of the Protect Role of Histamine on Intestinal Barrier Function

目的探讨组织胺对肠黏膜屏障的保护作用。方法从颈静脉插管放血加十二指肠灌注大肠杆菌制成失血感染模型。观察组织胺作用0.5,1,2,3 h后侵入肝脏和肠系膜淋巴结(MLN)大肠杆菌数,免疫球蛋白IgA分泌量。结果组织胺作用0.5,1,2,3 h,侵入肝脏大肠杆菌数分别为1 523,480,153,76个。侵入MLN大肠杆菌数分别为2806,763,264,140个,对照组肝脏和MLN大肠杆菌数分别为1 368,2 683,组织胺作用1 h后的实验组与对照组相比均具有统计学意义。(P<0.05)。IgA分泌量分别为1.201,20.158,32.674,43.520,对照组为1.367。组织胺作用1 h后的实验组与对照组相比均具有统计学意义。(P<0.05)。结论一定剂量和作用时间的组织胺有修复肠黏膜,增强肠黏膜屏障功能,抑制细菌移位的作用。

Objective Obsereation of the function role of histamine on intestinal barrier. Methods 1 × 10^-6 mol / L doses histamine or saline were injected subcutenously in rats subjected to hemorrhage combined with infection or controls.0.5,1,2,3 histamine perfusion observed in the hours after the invasion of the liver and MLN bacteria, immunoglobulin IgA secretion. Results In 0.5,1,2, 3 histamine-hour infusion,the amount of E.coli invasion of the liver were 1 523,480,153,76.MLN invasion of the amount of E.coli were 2 806,763,264,140,in the control group MLN and liver were 1 368,2 683,and the role of histamine 1 hour after the experimental group and control group were statistically significant. （P 〈 0.05）. IgA secretion were 1. 201,20.158,32. 674,43.520,1. 367 for the control group. The role of histamine 1 hour after the experimental group and control group were statistically significant. （ P 〈 0.05）. Conclusion A certain time and dose of Histamine has the effect on mucesal repair and the enhance intestinal mucosal barrier function,inhibit bacterial translecation.