降脂酮对CCl_4诱导的脂肪肝的保护作用

Effects of Jiangzhitong on Carbon Tetrachloride-induced Fatty Liver

目的从含有黄芩、枸杞子、山楂等6味中药的复方制剂中提取有效部位:降脂酮,观察降脂酮对四氯化碳(CCl4)诱导的脂肪肝的影响及其作用机制。方法建立CCl4脂肪肝模型,分别用低剂量、中剂量、高剂量降脂酮进行治疗,观察各组大鼠肝组织中超氧化物岐化酶(SOD)、丙二醛(MDA)、羟脯氨酸(PyH)、游离脂肪酸(FFA)的活性或含量。结果与正常对照组比较,CCl4诱导的脂肪肝模型对照组大鼠SOD活性明显降低(P<0.01),MDA含量明显升高(P<0.01)、肝组织匀浆游离脂肪酸、羟脯氨酸明显升高(P<0.01);与CCl4模型对照组比较,低、中、高剂量降脂酮给药组SOD活性明显升高(P<0.05);中、高剂量降脂酮给药组MDA含量明显降低(P<0.05);中、高剂量降脂酮给药组肝组织匀浆FFA有明显差异(P<0.05),高剂量降脂酮给药组Hyp有明显差异(P<0.05),余各剂量组统计学上差异不明显(P>0.05)。结论降脂酮能够减轻CCl4诱导的肝脏脂肪病变程度。其机制可能与降脂酮所具有的抗氧化的作用有关。

Objective Jiangzhitong, the active ingredients of compound preparation of 6 Oriental herbs （baikal skullcap root, Lyeii Fruetus,Crataegi and so on）, was extracted. The protective effects and the possible mechanism of jiangzhitong were explored on CCl4- induced fatty liver in rats. Methods Rats were treated with carbon tetraehloride to induce fatty fiver. After the model was established,rats were treated with jiangzhitong. The following parameters were detected in all groups： the activities of SOD,the contents of MDA, and the elevels PyH, FFA of liver tissues. Results jiangzhitong improved the CCl4-indueed decrease of hepatic SOD activities, it also inhibited the formation of MDA, PyH and FFA. Conclusion The present investigation was to verify the hepatoproteetive effect of jiangzhitong. The hepatoproteetive action is likely related to its potent antioxidative.