摘要
目的检测结肠癌患者血清中胰岛素样生长因子-1(Insulin-like Growth Factor-1,IGF-1)的含量及组织P53的表达并探讨其在结肠癌中的临床意义。方法应用双位点免疫放射测定法(IRMA)及SP免疫组化染色对20例不同分期结肠癌及15例正常对照的血清IGF-1及组织P53的表达进行检测。结果结肠癌患者血清中IGF-1含量较正常对照组有显著差异(P<0.05),两组间P53的表达有明显差异(P<0.01),不同IGF-1水平P53阳性表达差异有统计学意义(P<0.01)。结论结肠癌患者血清IGF-I水平及组织P53表达密切相关,提示IGF-I和P53可能参与了结肠癌的发生发展过程,可望作为结肠癌诊治的重要检测指标。
Objective To quantify the level of insulin-like growth factor-I（IGF-I） in serum and tissue P53 expression and investigate its clinical significance in colon cancer.Methods IGF-1 immunoradiometric assay（IRMA） and SP immunohistochemical examination was set up and used it to determine serum IGF-1 level and tissue P53 expression respectively in patients with colon cancer（ n = 20） and healthy controls （ n = 15）.Results There was high level of IGF-1 in patients with colon cancer than in healthy controls （P 〈 0.05）, while difference in the tissue P53 expression between two groups was significant（ P 〈 0.01）.The difference in serum IGF-1 level and tissue P53 expression was statistically significant（ P 〈 0.01 ） Conclusion Our study suggests teat there is significant role of serum IGF-1 level and tissue P53 expression in tumorogenesis and tumor progression in colorectal cancer and can prove to be key marker in the diagnosis and prognosis of colorectal cancer.
出处
《中国实验诊断学》
2008年第12期1546-1548,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助项目(NO.30271280)
