肾衰康2号抗肾纤维化及其尿毒素清除作用的研究

Prevention of renal fibrosis and clearance of uremia toxin by shenshuaikang 2

目的观察肾衰康2号对5/6肾切除大鼠所致慢性肾衰肠道清除尿毒素及其延缓肾脏纤维化的作用。方法将5/6肾切除大鼠分为肾衰康治疗组和对照组,并设假手术组为正常对照。普通喂养10周后检测各组的血清及粪便尿素氮、肌酐,并留取肾组织作病理,免疫组化法检测肾组织内纤维连接蛋白(FN)的表达水平。结果肾衰康2号治疗组血清尿素氮、肌酐较对照组下降(P<0.05),粪便尿素氮、肌酐水平较对照组升高(P<0.05),病理切片显示治疗组残余肾小球体积、直径与对照组相比明显减小(P<0.05),肾小球系膜细胞、基质的增生程度亦明显减轻(P<0.05),免疫组化显示,治疗组肾小球内FN的沉积比对照组减少(P<0.05)。结论肾衰康2号有延缓肾纤维化,促进尿毒素排泄的作用。

Objective To observe the clearance of uremia toxin through intestinal tract by shenshuaikang 2 and its effect on the prevention of the renal fibrosis in chronic renal failure rats with 5/6nephronectorny. Methods Subtotally nephrectomized rats were randomly assigned to shenshuaikang 2 lreatrnent group and no, treatment group. Sham-operation rats served as a normal control group. BUN, Cr, stool BUN, and Cr were measured at 10th week following the operation. Renal pathology changes were evaluated at 10th week Irnrnunohistochernistry was used to examine the expression of fibroneetin in glornerulus. Results Shenshuaikang 2 not only increased clearance of BUN and Cr through the intestinal tract, decreased BUN, Cr （P〈0.05）, but also ameliorated rnesangial tissue proliferation, reduced fibronectin deposition, and relieved compensatory hypertrophy in remnant renal glomerulus of 5/6 nephrectomized rats （P〈0.05）. Conclusion Shenshuaikang 2 can delay the progression of renal fibrosis and increase clearance of uremia toxin.