摘要
目的建立测定赶黄草中没食子酸含量的反相高效液相色谱方法。方法Hypersil BDS-C18色谱柱，流动相为乙腈-0．1％磷酸水溶液（10：90），检测波长为270nm，流速为0．8mL·min^-1。结果赶黄草中没食子酸与其他成分得到很好的分离，测定没食子酸含量的线性范围为0．0040～0．1006mg·mL^-1，r=0．9998，平均回收率为99．22％。结论本方法简单、快捷、重现性好，适用于赶黄草中没食子酸的含量测定。
Objective To establish an RP-HPLC method for the determination of gallic acid in Penthorum chinense Pursh. Methods Gallic acid was analyzed by RP-HPLC on a Hypersil BDS-C18 column and detected at 270 nm. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid （10 ： 90） at 0. 8 mL · min^-1. Results Gallic acid was well separated from other compounds in Penthorum chinense Pursh. The calibration curve was linear at 0. 004 0 0. 100 6 mg ·mL^-1 of gallic acid with correlation coefficient 0. 999 8. The average recovery of gallic.acid was 99.22%. Conclusion The method is fast, reliable, accurate, and suitable for the analysis of gallic acid, and for the quality control in Penthorum chinense Pursh.
出处
《中南药学》
CAS
2008年第6期717-719,共3页
Central South Pharmacy
基金
湖南省科技厅国际合作重点项目(2007wk2003)
