乙二醛对小鼠抗氧化系统的影响

Effects of glyoxal on anti-oxidative system in mice

目的:通过观察乙二醛(glyoxal)急性染毒小鼠全血及组织中超氧化物歧化酶(superoxide dismutase SOD)谷胱甘肽过氧化物酶(glutathione peroxidase GSH-Px)、巯基含量(T-SH、NP-SH)及脂质过氧化物(Lipid Peroxidation,LPO)含量的变化,探讨乙二醛对抗氧化系统的影响及毒作用机制。方法:实验用昆明种小鼠40只,分成4组。实验组分三组,腹腔注射乙二醛水溶液,剂量分别为1.3 mmol/kg(1/8 LD50)、2.6 mmol/kg(1/4 LD50)及5.2 mmol/kg(1/2 LD50),对照组注射等量生理盐水。急性染毒后24h,采血并取心、肝、脑及肾组织。全血及组织中GSH-Px的活力测定,采用二硫双硝基苯甲酸(DTNB)法;SOD活力的测定用亚硝酸盐法。血清及组织中的LPO(主要为MDA)含量测定,用硫代巴比妥酸(TBA)法;全血及组织中总巯基(T-SH)和非蛋白巯基(NP-SH)含量测定,用Ellmans法,蛋白巯基(PB-SH)含量可由T-SH减去NP-SH求得。结果:各染毒组脑及高剂量组肝的LPO含量升高,与对照组比较差异具有显著性(P<0.05或P<0.01)。低剂量组肝及高剂量组脑的SOD活力增高;各剂量组的全血和肾脏及中、低剂量组肝脏GSH-Px活力下降;各剂量组全血及高剂量组脑的总巯基及蛋白巯基含量增加,高剂量组脑、肾及中、低剂量组肝脏非蛋白巯基含量下降,均与对照组相比差异显著(P<0.05或P<0.01)。结论:小鼠脑及肝组织对毒物诱发的自由基的高度敏感,易致脂质过氧化损伤;血、肝及肾中GSH-Px为接触乙二醛的敏感指标。

objective ： To study the effects of glyoxal on anti-oxidative system in mice and its mechanism of toxicity through measuring the content of SOD （Superoxide Dismutase）, GSH-Px （Glutathione Peroxidase）, T-SH （Total Sulfhydryl） , NP-SH （Non- Protein Sulfhydryl） and LPO （Lipid Peroxidation） in the blood and tissue in mice treated with glyoxal. Method： This study use 40 mice of KunMing species, which are divided into 4 groups evenly on random. 1 is negative control group treated with normal saline, other 3 is experiment groups treated with glyoxal of 1.3mmol/kg （1/8 LD50） , 2. 6mmol/kg （1/4 LD50） and 5.2mmol/kg （ 1/ 2 LD50） separately. The mice were taken blood, heart, liver, brain and kidney after they were treated with glyoxal for 24 hours. The activity of GSH-Px in blood and tissue were measured by DTNB method; The content of T-SH and NP-SH in blood and tissue were measured by Ellmans, and the content of PB-SH can be got from T-SH subtracting NP-SH. The activity of SOD in blood and tissue were measured by nitrite method. The content of LPO （especially MDA） in serum and tissue was measured by TBA method. Results ： The content of LPO in various dose groups in brain and in high dose group in liver of mice is significantly higher than that of the control group （ P 〈 0. 05 or P 〈 0. 01 ）. The activity of SOD in liver and brain of high dose groups increases. The activities of GSH-Px significantly decrease in blood and kidney at any dose groups, in liver high and middle dose groups decrease. The content of T-SH and PB-SH increases in blood and brain of high dose groups. The content of NP-SH decreases in brain and kidney of high dose groups and in liver of middle and low dose groups. Comparing with control group, the difference is notable （P 〈0. 05 or P 〈0. 01 ）. Conclusion ： The liver and brain of mice have high sensitivity to free radical caused by Glyoxal , and they were damage easily by Lipid Peroxidation; The activity of GSH-Px in blood , liver and kidney is the sensitive index expose to glyoxal.