双相障碍患者血管紧张素转换酶基因多态性研究

Research of the polymorphisms of angiotensin-converting enzyme gene in bipolar disorder patients

目的:探讨双相障碍与血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性之间的相关性。方法:按照《中国精神障碍分类和诊断标准》(CCMD-3)的双相障碍的诊断标准选择研究组64例、健康对照组100例;每例研究对象抽取静脉血提取DNA,应用聚合酶链式反应(PCR)及限制性片段长度多态性(RFLP)技术检测ACE基因的I/D多态性,采用病例-对照的关联分析方法对基因型和等位基因频率进行分析。结果:研究组和对照组的ACE基因型符合Hardy-Weinberg平衡法则;两组间ACE基因型和等位基因频率分布均无显著性差异。结论:TPH基因型和等位基因频率分布与双相障碍无关联性。

Objective： To explore the association between I/D polymorphisms of the angiotensin - converting enzyme gene and bipolar disorder.Methods：64 patients were recruited according to the diagnosis criteria of bipolar disorder in CCMD- 3, and 100 healthy persons in the control group; Venous blood was taken from all individuals into the ACD - containing tube. After DNA isolation, the ACE gene were determined by the polymerase chain reaction - restriction fragment length polymorphism （PCR - RFLP） technique. The ease - control association analysis was adopted to analyze the frequencies of genotype and alleles between the patients and the healthy persons. Results： The values of the genotypes of the both groups consistent with the Hardy - Weinberg equilibrium; The distribution of genotypes or alleles of ACE genes had no significant difference between the two groups （ P 〉 0.05）. Conclusion： No association between the polymorpliisms of ACE gene and the bipolar disorder.