应用结合bFGF的壳聚糖导管修复大鼠坐骨神经损伤的实验研究

Repair of Sciatic Nerve Gap of Rats with Chitosan Tube Combined with Basic Fibroblast Growth Factor

目的观察用结合碱性成纤维细胞生长因子(bFGF)的壳聚糖导管促进周围神经损伤再生的情况。方法实验组10只成年Wistar大鼠造成10 mm坐骨神经缺损后,以结合bFGF的壳聚糖导管作桥梁桥接神经两断端,以假手术组和单纯损伤组(造成10 mm坐骨神经缺损后,不加以任何干预措施)各10只大鼠为对照。术后3个月,通过大体观察、形态学及电生理检查观察损伤神经的再生情况。结果术后3个月,实验组大鼠新生的神经纤维已通过缺损部位,手术局部未出现明显的炎症反应,各项指标明显优于单纯损伤组。结论结合bFGF的壳聚糖导管对缺损的坐骨神经修复具有良好的桥梁作用和促进神经生长的作用。

Objective To observe the effect of the chitosan tube combined with basic fibroblast growth factor （bFGF） on inducing nervesax on;regeneration of rats with peripherial nerve injury. Methods A novel chitosan tube combined with bFGF was developed and used to suture, the 10 milimeter long right sciatic nerve gap of 10 rats, single injury group （10 rats） were the control with sciatic nerve injury alone, and other 10 rats were assigned to sham group. Immunohistochemistry and electrophysiology study had been done to observe the effect of repairing. Results 3 months after operations, the sciatic nerve gap were repaired by the regeneration nerve in the experiment group. And there was no evident inflammation in the defects. Conclusion The chitosan tube combined with bFGF can induce the sciatic nerve to regenerate.