锁阳黄酮对老年大鼠的抗疲劳作用

Anti-fatigue Effect of Cynomorium Songaricum Flavone on Old Rats

目的探讨锁阳黄酮对老年大鼠运动耐力和抗氧化作用的机制。方法50只老年雄性Wistar大鼠随机分为对照组(不游泳、不给药)、单纯游泳组(游泳+生理盐水灌胃)、游泳+给药组(分为锁阳黄酮20 g/kg体重、10 g/kg体重和5 g/kg体重灌胃组),共5组,每组10只。采用循环水流自由游泳,动物每天游泳1次,每次30 min,连续10 d,第11天进行运动耐力测试;用紫外分光光度计和试剂盒检测血液中单胺氧化酶(MAO)和谷胱甘肽过氧化物酶(GSH-px)活力。结果三个游泳+给药组动物体重比单纯游泳组分别增长12.9%、80.6%和200%;沉下前游泳时间比单纯游泳组增加19.4%、29%和48.4%;每只动物沉下次数(次/10 min)减少17.8%、43.2%和72.9%;总游泳时间延长2.8%、7%和29.6%;MAO比单纯游泳组降低60%、69.8%和80.5%,差异均有非常显著性意义(P<0.01);GSH-px增加465.3%、563.8%和635%,差异亦有非常显著性意义(P<0.01)。结论锁阳黄酮具有增强运动耐力、提高机体抗氧化、抗疲劳的作用。

Objective To study the anti-fatigue effect of Cynomorium songaricum flavone （CSF）on old rats. Methods 50 old male Wistar rats were randomly divided into the control group （no swimming, no drugs）, single swimming group （swimming, given sa line by gavage）, and three swimming plus CSF groups （swimming, given CSF 20 g/kg weight, 10 g/kg weight and 5 g/kg weight respectively）, total five groups with 10 animals in each group. Animals swam with free swimming in endless cycles water 30 minutes once every day for 10 days. The level of monoamine oxidase （MAO） and glutathione peroxidase （GSH px） in blood of animals were examined by MAO kit, GSH-px kit and ultraviolet speetrophotometer at 11th day. Results Compared with single swimming group, the body weights of swimming plus CSF groups increased significantly by 12. 9%, 80. 6% and 200% respectively （ P 〈0. 01）; swimming times before sinking increased by 19. 4%, 29% and 48. 4%; sinking times of each rat （time/10 min） decreased by 17.8%, 43.2% and 72.9% ; total swimming times increased by 2.8%, 7% and 29.6% respectively （ P 〈0.01） ; MAO decreased by 60%, 69.8% and 80.5%, GSH-pxincreased by 465.3%, 563.8% and 635% respectively （P〈0.01）.Conclusion CSF shows anti-oxidative and anti-fatigue properties and can be given as prophylactic/therapeutic supplements for increasing antioxidant enzyme activities and preventing lipid peroxidation during strenuous exercise.