结球甘蓝游离小孢子培养技术的初步研究

Prelimary Study on Microspore Culture Technique of Cabbage

以4个不同的结球甘蓝品种为试材进行游离小孢子培养,对影响小孢子胚胎发生的几个因子进行探讨。研究结果表明,不同基因型对小孢子的培养出胚数有影响,QS13,321,325优化的培养基为NLN-13+0.5mg/LNAA+0.05mg/L6-BA,培养密度1×105个/mL,且尽量选择初花期进行取材培养。

Four cabbage cultivars were chosen for isolated microspore culture, and several factors were studied, including genotype, hormone, culture density, culture period. Different genotypes have different influence on the embryogenesis. For genotype QS13, 321,325, the optimum medium is NLN-13＋0.5 mg/L NAA＋0.05 mg/L 6-BA, culture density is 1×10^5 / mL, and it is better to be cultured in the earlier flowering stage.