单个黄原胶分子的样品制备与原子力显微镜检测

Single xanthan molecule preparation and atomic force microscopy observation

本文发展了一种新的黄原胶分子样品制备方法——多步旋涂法,并采用此方法在4种原子力显微镜研究中常用的衬底(裸云母、3-氨丙基三已氧基硅烷处理的云母、镍离子处理的云母和高序热解石墨)上制备了分散的单个黄原胶分子。利用原子力显微镜对单个黄原胶分子高度的检测和分析结果显示衬底的电学特性和亲疏水性质对黄原胶分子的形貌有显著影响。

A newly developed sample preparation technique termed multi-step spin-coating method was used to prepare well-dispersed xanthan molecules on bare mica, 3-aminopropyl triethoxysilane treated mica, nickel ion treated mica and highly oriented pyrolytic graphite, which are widely used as supporting surfaces in the atomic force microscopy （AFM） study. The substrate effects on conformation of single xanthan molecules were also investigated. We believe that this study is of great importance for the in-depth studies on xanthan molecules at the single-molecular level.