H22荷瘤小鼠不同阶段及证候的甲状腺上下调高表达基因

Up or Down-regulated High Expressed Genes in Different Stage from Thyroid of H22 Tumour Mice

目的:揭示荷瘤小鼠甲状腺不同阶段上下调高表达基因特征。方法:采用荷瘤小鼠及其标准化四诊及辨证方法,以及GeneChip Mouse Exon 1.0 ST Array等技术,检测H22荷瘤小鼠早期邪毒壅盛证和气虚证、中期阳气虚证、中晚期气阴阳虚证4个证候甲状腺上下调基因,重点关注那些高表达量的基因。结果:早期邪毒壅盛证和气虚证同时上调高表达基因1个即Ifi27,下调高表达基因2个即AY036118和Dub2a;中期阳虚证上调高表达基因25个,如Clec2g和Probe号6809296的基因等,下调高表达基因5个即Klklb27,Crispl,Fas,H2-Ea和GOs2;中晚期气阴阳虚证上调高表达基因1个即Miff,下调高表达基因3个即Spare,Col4al和Col3al。结论:部分基因表达量改变及功能特征呈现一定的趋势,如中期阳气虚证上调高表达基因数量明显多于早期及中晚期,且与细胞构造、运动、增殖、凋亡相关基因较多,推测可能与肿瘤发生相关,以上绝大部分基因,以往在甲状腺生理与病理研究中较少涉及。这些基因具体功能如何,及甲状腺在肿瘤发生中所起的作用,有待进一步研究。

Objective：To reveal the character of highly expressed Genes in different stage from the thyroid of H22 Tumour Mice.Methods：By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed thyroid gene expression about poisonous pathogenic factors syndrome and asthenia of qi syndrome on earlier period tumour mice.Highly expressed highly are analyzed in the paper.Resuhs：in early period,there are 1 up-regulated gene Ifi27 and 2 down-regulated high expressed genes, namely AY036118 and Dub2a in middle stage,25 up-regulated high expressed genes were Ktklb27, Crispl, Fas,H2-Ea and GOs2. In intermediate and advanced stage,there are 1 up-regulated and 3 down-regulated high expressed genes,the former namely Miff,the latter kind namely Sparc, Co 14al and Co 13al.Conclusion The expression and functional characteristics of some genes present certain ternds, for example,in middle stage,the up-regulated high expressed genes are much more than that in other two stages.These genes involved in cellular architecture,movement,proliferation,apoptosis may be related to Tumorigenesis.In these genes above,most genes were less researched in thyroid physiology and pathology.They function in thyroid and the thyroid function in Tumorigenesis expecting more study.