一种新来源天然冰片对CHL细胞的细胞毒性及染色体畸变作用研究

Study on Cytotoxicity and Chromosomal Aberration of A New Natural Borneol with Chl Cells

应用MTT法检测不同浓度的天然冰片对中国仓鼠肺成纤维(CHL)细胞的细胞毒性,并考察了天然冰片对CHL细胞的染色体畸变效应。发现MTT还原量、生成甲臢的光密度(OD)值与天然冰片之间存在剂量关系,24 h的50%细胞生长抑制浓度(IC50)为207.86μg·mL-1。染色体畸变试验剂量为200,100,50,25,12.5μg·mL-1,于加药(+/-S9mix)后24 h收获细胞,制备染色体。各剂量组的染色体畸变率<5%;空白及溶剂对照组的染色体畸变率均在正常范围内;而阳性对照剂诱发的染色体细胞畸变率>20%;各剂量组与溶剂对照组比较差异无统计学意义(P>0.05)。结果表明:天然冰片无诱发CHL细胞染色体畸变作用。

To Study cytotoxicity of natural borneol with Chinese Hamster Lung（CHL）Cells by MTT method. At the same time, the effects of natural borneol on chromosomal aberration were also studied. The results showed that the reduction of MTT was decreased, and the amount of formazane produced depended on the concentration of natural borneol. The 50% inhibition （IC50） of natural borneol for 24 hours, was achieved at 207.86μg·mL^-1 （r〉0.9）. By the chromosomal aberration test, CHL cells were treated with natural borneol at the concentration of 200, 100, 50, 25, 12.5 （μg·mL^-1） After 24 hours （in the presence of S9mix or not respectively）, the proportion of chromosomal aberration was always less than 5% with the alteration of dose. There was not significant difference between these results obtained from the Borneol assay and the negative control assay（P〉0.05）, and however, the frequency of chromosome aberration in the positive control assay were obviously higher than 20%. Conclusion： Natural Borneol was not display the potential to lead to the chromosomal aberration of CHL cells.