摘要
研究了曼地亚红豆杉枝条中紫杉醇的超声浸提，通过液液萃取对粗提物进行二次纯化，用高效液相色谱检测紫杉醇的含量。试验确定紫杉醇的最佳浸提工艺条件为：浸提时间：90min（三次浸提，30min／次）；温度：50℃；原料粒径：0．180—0．120mm。最佳工艺条件下用10．0g原料提取得到的0．0465g浸膏中，紫杉醇质量浓度为6．182％。
The elementary extracting technology of taxol from the branches of Taxus media was studied with the method of ultrasonic. Then, the crude extracts of taxol were purled ulteriorly with liquid-liquid extraction and the content of taxol was detected by HPLC. Then we confirmed the optimal extracting conditions as follows： the time of extraction was 90min（30min per time） ; the temperature was 50~C and the granularity of raw material was 0. 180 -0. 120ram. Following the optimal conditions, 10. 0g raw materials were extracted and 0. 0465g crude extracts were received, which the content of taxul reached 6. 182%.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2008年第12期1637-1640,共4页
Chemical Research and Application
基金
中南大学研究生教育创新工程项目(1343-77330
1343-77228)
关键词
曼地亚红豆杉
超声浸提
紫杉醇
高效液相色谱
taxus media
ultrasonic extraction
taxol
high performance liquid chromatography
