GnRH-A抗原主动免疫雄兔对血液Fe、Cu、Zn的影响

Effects of GnRH-A Active Immunocastration on Blood Fe,Cu and Zn Contents in Rabbits

目的:探讨促性腺激素释放激素类似物-A(GnRH-A)主动免疫时动物血液微量元素的变化规律,为深入研究GnRH免疫去势的作用机理提供科学依据.方法:用自制的GnRH-A抗原乳剂1.0 mL对兔子进行皮下注射,美国热电SOLAAR S4型原子吸收光谱仪测定Fe、Cu、Zn.结果:Zn的含量在第4周明显高于免疫去势前的水平(P<0.05),Cu和Fe与免疫去势前无显著差异(P>0.05).结论:GnRH-A主动免疫对雄兔血液微量元素无明显影响.

Objective： To explore the changes of blood microelements contents of （iron, cupper and zinc） in male rabbits When they are immunized actively by injecting gonadotropin releasing hormone analogues （GnRH- A）, and to provide researchers some scientific basis for study of the effects of GnRH immunization castration and mechanism profoundly in animals. Methods： 1.0 mL GnRH - A antigen emulsion were subcutaneously injected into experimental rabbits, contents of iron, cupper and zinc were measured with SOLAAR S4 atomic absorption spectrometer. Results： Zinc contents in the first 4 weeks was significantly higher than the level before the immune castration （P 〈 0.05）, Cupper and iron contents had no- significantly difference compared with the level before immunocastration （P 〉0.05）. Conclusion： GnRH- A active immunization for male rabbit had no remarkable effects on microelements in male rabbit.