不同浓度血红蛋白对小鼠骨髓瘤细胞体外培养的影响

Effects of different Concentrations of Hemoglobin on Mice SP2/0-Ag14 Growth in vitro

用含不同浓度的牛源性血红蛋白细胞培养液培养小鼠骨髓瘤细胞，通过细胞计数绘制生长曲线，计算最大增殖浓度和倍增时间。并进行比较分析．结果表明：当培养液的血红蛋白浓度在1-5rag／dL时小鼠骨髓瘤细胞生长较好，细胞最大增殖浓度在1．2×10^6／mL以上，倍增时间小于20h，当培养液的血红蛋白浓度为6mg／dL时细胞生长抑制，细胞最大增殖浓度和倍增时间为6．7×10^5／mL和21．6h．由此可见，培养液中血红蛋白浓度≤5mg／dL时不影响细胞生长，达到6mg／dL时抑制细胞生长．

SP2/0 - Ag14 cells were seeded in cell cultures which contained the different concentrations of hemoglobin from bovine blood. By means of accounting the number of SP2/0 - Ag14 cells, the growth curves were made and the Max proliferation concentration and population doubling time were analyzed. The results showed that at 1- 5 mg/dL concentration of hemoglobin, the SP2/0 - Ag14 cells grew well, and the Max proliferation concentration was more than 1.2 ×10^6 cell/mL, and the population doubling time appeared before 20 hours. When in cell culture the concentration of hemoglobin was over 6 mg/dL, the SP2/0 - Agl4 cell growth was arrested, the Max proliferation concentration were 6.7 ×10^5 cell/mL and the population doubling time was 21.6 hours. The concentration of hemoglobin which was lower than 5 mg/dL failed to affect the SP2/0 - Ag14 cell growth. However, one reaching 6mg/dL could restrict the growth of SP2/0 - Ag14.