多药耐药大肠埃希菌耐药基因谱及菌株亲缘性分析

Resistance Gene Spectrum and Phylogenetic Analysis in Multidrug-resistant Strains of Escherichia coli

目的了解多药耐药大肠埃希菌(ECO)分离株耐药基因谱与亲缘性。方法采用聚合酶链反应(PCR)检测20株ECO 34种耐药基因,并作聚类分析。结果20株中TEM、SHV、CTX-M-1群、OXA-1群、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3″)-Ⅰr、mtB、dfrA1、dfrA12、dfrA17s、ul1c、atB和cmlA基因阳性率分别为95%、55%、40%、10%、75%、40%、15%、5%、10%、5%、75%、90%、15%和35%,聚类分析示存在克隆传播现象。结论TEM、SHV、CTX-M-1群、aac(3)-Ⅱ、aac(6′)-Ⅰb、dfrA17、sul1和cmlA基因检出率高,ECO可导致克隆传播医院感染。

OBJECTIVE To investigate the resistance gene spectrum and phylogenetic analysis in multidrug-resistant strains of Escherichia coll. METHODS The 34 kinds of resistant genes were detected by polymerase chain reaction and cluster analysis. RESULTS The positive rates of TEM, SHV, CTX-M-1 group, OXA-1 group, aac（3）-Ⅱ , aac（6＇）-Ⅰb, ant（3＂）-Ⅰ, rmtB, dfrA1, dfrA12, dfrA17, sull, catB and cmlA were 95%, 55%, 40%, 10%, 75%, 40%, 15%, 5%, 10%, 5%, 75%, 90%, 15% and 35%, respectively. There was clone transmitted phenomenon. CONCLUSIONS There are very high positive percentages of TEM, SHV, CTX-M-1 group, aac（3）-Ⅱ , aac（6%）- Ⅰ b, dfrA17, sull and cmlA genes in multidrug-resistant strains of E. coli. The multidrug-resistant strains of E. coli can induce clone transmitted hospital infection.