普罗威登斯菌属β-内酰胺酶的分类检测

Classification and Detection of β-Lactamases Produced by Providencia spp

目的研究普罗威登斯菌属所产各种β-内酰胺酶分布情况。方法采用头孢硝噻吩试验检测19株普罗威登斯菌属所产β-内酰胺酶,多底物纸片法(协同法、拮抗法)、AmpC酶检测法、碳青酶烯酶分类检测其所产各种β-内酰胺酶。结果19株普罗威登斯菌属头孢硝噻吩试验均为阳性,β-内酰胺酶总检出率为100.0%,其中单独产广谱酶、超广谱β-内酰胺酶(ESBLs)、碳青酶烯酶分别为8株占42.1%、8株占42.1%、3株占15.8%,持续高产AmpC酶1株,未检出持续高产AmpC酶+ESBLs复合产酶菌。结论普罗威登斯菌属产β-内酰胺酶率很高,产3种酶,以广谱酶、ESBLs为主。

OBJECTIVE To study the classification the β-lactamases produced by Providencia spp. METHODS To detect the β-lactamases produced by 19 strains of Providencia spp with nitrocefin test, a multi-disk test （synergy test, antagonized test for the inducible AmpC β-lactamases （IABLs）, AmpC β-lactamases （ABLs） phenotype test and extended-spectrum β-lactamases （ESBLs） screening test, carbapenem-hydrolyzing β-lactamases （CHBLs）. RESULTS The nitrocefin tests of all of 19 strains were positive, producing the β-lactamases（100. 0%）; eight strains produced broad-spectrum β-lactamases （BSBLs, 42. 1 %） and ESBLs （42. 1 %）, respectively, one strain produced overexpression of AmpC, 3 Strains produced CHBLs. CONCLUSIONS The rate of β-lactamases produced by the 19 strains of Providencia spp is very high, there are three kinds of β-lactamases, mainly BSBLs and ESBLs.